Drive performance in transient protein expression with the eviCHO Expression Kit
Chinese Hamster Ovary (CHO) cells are the gold standard for recombinant protein expression, offering robust process performance and scalability. However, the complexity of transient protein expression workflows in early discovery often requires a streamlined, reliable, and high-performance solution to meet the demands of research-grade, recombinant protein production, such as in antibody expression applications.
Integrate evitria’s expertise in your research lab – choose science over troubleshooting
The eviCHO Expression Kit provides an effective solution for transient protein expression – Swiss quality out of the box. Whether you are optimizing workflows, scaling transient protein production, or accelerating discovery, the eviCHO Expression Kit delivers the reliability and performance you need to achieve consistent results.
- Optimized for performance: Each element of the kit is designed to deliver high protein expression levels with minimal effort and easy protocols.
- Scalable and flexible: Suitable for a wide range of applications, from small-scale research to larger-scale transient protein production.
- CHO expression system: Robust CHO-K1 cells that recover rapidly in eviSTAR medium after thawing, with a doubling time of approximately 16 hours. They maintain high protein expression levels across multiple passages.
The ready-to-use eviCHO Expression Kit: What is in it?
The eviCHO Expression Kit includes all components that are needed to master your experiments:
| Component | Description | Amount |
|---|---|---|
| eviCHO | evitria CHO-K1 cells recover rapidly in eviSTAR medium after thawing. They have a doubling time of approximately 16 hours and maintainhigh protein expression levels over numerous passages. | 1.5 mL (1x107 cells mL-1) |
| eviSTAR | Chemically defined, serum-free, protein-free, and animal origin-free maintenance media which is specifically designed for the maintenance of eviCHO cells. | 1 L |
| eviFECT | Chemically defined, serum-free, protein-free, and animal origin-free transfection media which is specifically designed for the transient transfection of eviCHO cells. | 1L |
| eviFEED | Chemically defined, serum-free, protein-free, and animal origin-free feed media which is engineered for high-performance transient transfection of eviCHO cells. | 200 mL |
| eviACT | Highly efficient transfection reagent designed for high-producing eviCHO cells. It leads to high protein titers in eviCHO cells without a complexation step. | 15 mL |
| eviBOS | The eviBOS enhancer, in combination with the eviACT transfection reagent, results in maximum protein titers in eviCHO cells. | 5 mL |
| eviGLN | eviGLN is a dedicated supplement that supports robust growth of eviCHO cells. It provides a stabilized glutamine source to maintain optimal cell metabolism and viability throughout cultivation. | 25 mL |
| eviCOP | eviCOP is an animal-origin free reagent and can be added to the eviFEED to enhance protein production in eviCHO cells. | 2 mL |
| eviDNA | CMV-based positive control vector designed for efficient transfection and expression in eviCHO cells. It includes the human IgG heavy and light chains at a 1:1 ratio and is supplied at 1 mg/mL. Each vial provides enough material to support transfections of up to 50 mL. | 400 µL |
How it works: Transient protein expression with eviCHO
Want to learn more about the eviCHO Expression Kit for protein expression?
Please note: The eviCHO Expression Kit is for research use only - not for human or animal use. It is not for use in diagnostic procedures. Evonik acts solely as a distributor of products from evitria and has no responsibility or involvement in the quality assurance, quality control, testing, or release of the products as well as product safety and regulatory compliance.
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Frequently asked questions (FAQ)
To achieve optimal protein expression, it is recommended not to use the cells beyond 20 passages.
The recommended harvest time for protein expression using eviCHO cells is variable and dependent on the particular protein being expressed. Generally, the optimal time for harvesting is between 5 and 9 days after transfection, though some proteins may need to be harvested earlier or later.
A complexation step between the transfection reagent and plasmid DNA is not required.
The eviACT transfection reagent was specifically developed for eviCHO cells. To achieve maximum titer, the eviACT transfection reagent should be used in combination with other components of the eviCHO Expression Kit as defined in the protocol.
For optimal results, feeding should ideally occur within 2 to 4 hours post-transfection.
Only a single feeding step is required throughout the entire process.
When working with the eviCHO transient expression systems, CMV promoter-based vectors such as eviDNA are an excellent choice due to their proven ability to achieve high expression levels.
For optimal expression results with the eviCHO expression system, the corresponding media and feed should be used (eviSTAR, eviFECT and eviFEED)
To ensure optimal cell growth, we recommend subculturing eviCHO cells when they reach early log-phase, which typically corresponds to a density between 4 × 10⁶ and 5 × 10⁶ viable cells mL-1. This stage is usually reached every 2 to 3 days.
To achieve the highest possible titer, it is recommended to use 7.5 µg plasmid DNA per mL of transfection medium. Depending on the protein, the DNA concentration can be reduced.
To optimize eviCHO cell culture performance and viability:
- Maintain optimal incubator conditions: Strictly control temperature, CO2 levels, shaking rate, and humidity.
- Handle medium carefully: Avoid repeatedly warming the medium; only pre-heat the volume immediately required.
- Minimize time outside the incubator: Promptly return cell cultures after manipulations to reduce exposure.
- Optimize passaging: Passage cells during their early log phase and avoid over-passaging, following a routine schedule every 2-3 days.
- Perform medium changes meticulously: Centrifuge the cell suspension at 300g for 5 minutes, carefully aspirate the old medium with a pipette, and gently resuspend the cell pellet in fresh medium. Assess cell viability again after 2 days. If the cells have not recovered sufficiently, discard the culture and thaw a new vial.
