PhytoChol® BioPharma: a phytochemical plant-derived cholesterol to optimize your cell culture
Supplementing a cell culture medium with cholesterol can improve cell culture outcomes or even be essential for some cell lines. Chemically defined cell culture media that do not use fetal bovine serum (FBS) and natural lipids often require the addition of cholesterol to help improve cell growth, protein production, virus production and infectivity of produced viruses.
Evonik recognizes the need to provide animal-free cell culture solutions and offers PhytoChol® BioPharma for use in a wide range of cell lines including NS0 cell culture for protein production, culture of insect cell lines and culture of packaging cell lines for virus production.
Evonik’s PhytoChol® BioPharma is a tailor-made solution to help you optimize your cell culture formulations. As a pure powder, PhytoChol® BioPharma has been especially developed for use in cell culture media formulation. Before addition to the cell culture medium the powder can, for example, be dissolved in ethanol and formulated with other lipids and surfactants depending on intended use.
Whitepaper: Boosting biomanufacturing with optimized cell culture media - Comparing animal-derived cholesterol with synthetic PhytoChol® BioPharma
Cholesterol is a key ingredient in biopharma cell culture media. Supplementing media with cholesterol helps improve cell growth and enhance outcomes when producing therapeutic proteins, viral vectors and vaccines. Conventional cholesterol sourced from ovine wool raises concerns regarding variation in composition and purity.
In this white paper, we outline two studies that compare conventional animal-derived cholesterol with Evonik’s synthetic cholesterol PhytoChol® BioPharma. Our studies show that PhytoChol® Biopharma formulated with cyclodextrin or with fatty acids and surfactants performs similarly to an animal-derived cholesterol in NS0 and MSC cultures, respectively.
PhytoChol® Biopharma at a glance
From drug delivery to cell culture, our system solution PhytoChol® provides you with the following advantages:
- Completely non-animal derived
- Secure and stable supply
- Large-scale manufacturing
- High-purity, consistent quality
- Western-based manufacturing
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"If you are looking for ways to more efficient bioprocessing and improved performance, our innovative cell culture solutions will be of great value to you. Contact us to find out how we can support and meet your needs."
Kristina Klinker
Business Development Manager
